Fröhlich W
Zentralbl Bakteriol A. 1980 Jun;247(1):124-31.
Antibodies of Leptospirae were demonstrated in the sera of immunized rabbits by the indirect immunofluorescence test, only after a suitable dilution of the "anti-rabbit-gamma-globulin fluorescin conjugated" was carried out in a cross reference titration ("chessboard method"). A distinct crossreaction was observed when anti-icterohaemorrhagiae - and anti-bratislava-immunsera reacted with several serotypes out of the "interrogans complex". But only a weak crossreaction was found when both sera reacted with the serotyp patoc from the "biflexa complex". The evaluation of the indirect immunofluorescence test was complicated by a streaky background of fluorescence. The agargeldiffusion tests (Ouchterolony) exhibited four, three and two precipitation bands according to the conbination of antigens and immunsera.
仅在通过交叉参考滴定法（“棋盘法”）对“抗兔γ球蛋白荧光素结合物”进行适当稀释后，才通过间接免疫荧光试验在免疫兔血清中检测到钩端螺旋体抗体。当抗出血性黄疸型钩端螺旋体免疫血清和抗布拉迪斯拉发免疫血清与“问号状菌群”中的几种血清型发生反应时，观察到明显的交叉反应。但当两种血清与“双曲钩端螺旋体菌群”中的帕托克血清型发生反应时，仅发现微弱的交叉反应。间接免疫荧光试验的评估因荧光背景呈条纹状而变得复杂。琼脂扩散试验（奥克特洛尼试验）根据抗原和免疫血清的组合显示出四条、三条和两条沉淀带。
