钩端螺旋体病免疫荧光技术的一项贡献（作者译）
[A contribution to the technique of immunofluorescence in leptospirosis (author's transl)].
作者信息
Staak C, Schönberg A
出版信息
Zentralbl Bakteriol A. 1980 Jun;247(1):138-41.
PMID:7001804
Abstract
Mono- and polyvalent FITC-conjugates were prepared for detection of leptospires in artifically infected material. The organisms were concentrated by centrifugation and fixed onto glass slides after they had been mixed with serum or egg albumin. By this method, leptospires were detected down to a concentration of 10(2)-10(4) organisms/ml.
摘要
制备了单价和多价异硫氰酸荧光素（FITC）结合物，用于检测人工感染材料中的钩端螺旋体。将这些微生物通过离心浓缩，并在与血清或蛋清蛋白混合后固定在载玻片上。通过这种方法，能检测到低至每毫升10² - 10⁴个微生物浓度的钩端螺旋体。
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