Crosignani P G, Donini P, Lombroso G C, Donini S, Caccamo A, Trojsi L
Acta Endocrinol (Copenh). 1980 Oct;95(2):232-6. doi: 10.1530/acta.0.0950232.
A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.
报道了一种大规模制备适用于人类的部分去唾液酸人绒毛膜促性腺激素的方法。为了获得所需程度的去唾液酸化并避免修饰后的激素中存在酶，使用了偶联到琼脂糖4B上的神经氨酸酶。该制剂在体外（OAAD和SVW试验）显示有活性，其半衰期在大鼠中为13分钟，在人类中为75分钟。这种去唾液酸hCG被证明对闭经女性诱导排卵有效。在39个诱导周期中，发生了31次排卵和5次妊娠。
