Chang R C, Huang W Y, Redding T W, Arimura A, Coy D H, Schally A V
Biochim Biophys Acta. 1980 Oct 21;625(2):266-73. doi: 10.1016/0005-2795(80)90290-1.
Ten peptides were isolated from porcine hypothalami and structurally elucidated. These included four dipeptides Arg-Phe, Phe-Tyr, Val-Trp, and Tyr-Phe; a tripeptide Lys-Phe-Tyr; two tetrapeptides Gly-Lys-Val-Asn and Phe-Glu-His-Glu, a nonapeptide Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; a decapeptide Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe and a hexadecapeptide Phe-Leu-Gly-Phe-Pro-Thr-Thr-Lys-Thr-Tyr-Phe-Pro-His-Phe-Asn-Leu. The tetrapeptide Gly-Lys-Val-Asn, the nonapeptide, the decapeptide and the hexadecapeptide most probably represent artifact fragments of alpha- and beta-chains of porcine hemoglobin. The natural or synthetic Gly-Lys-Val-Asn and Phe-Glu-His-Glu had some growth hormone releasing activity while Val-Trp, Tyr-Phe and Lys-Phe-Tyr had slight prolactin releasing activity. The biological activities of other peptides have not been determined yet.
从猪下丘脑分离出十种肽并对其结构进行了阐明。其中包括四种二肽：精氨酸-苯丙氨酸、苯丙氨酸-酪氨酸、缬氨酸-色氨酸和酪氨酸-苯丙氨酸；一种三肽：赖氨酸-苯丙氨酸-酪氨酸；两种四肽：甘氨酸-赖氨酸-缬氨酸-天冬酰胺和苯丙氨酸-谷氨酸-组氨酸-谷氨酸；一种九肽：缬氨酸-缬氨酸-酪氨酸-脯氨酸-色氨酸-苏氨酸-谷氨酰胺-精氨酸-苯丙氨酸；一种十肽：亮氨酸-缬氨酸-缬氨酸-酪氨酸-脯氨酸-色氨酸-苏氨酸-谷氨酰胺-精氨酸-苯丙氨酸；以及一种十六肽：苯丙氨酸-亮氨酸-甘氨酸-苯丙氨酸-脯氨酸-苏氨酸-苏氨酸-赖氨酸-苏氨酸-酪氨酸-苯丙氨酸-脯氨酸-组氨酸-苯丙氨酸-天冬酰胺-亮氨酸。四肽甘氨酸-赖氨酸-缬氨酸-天冬酰胺、九肽、十肽和十六肽很可能是猪血红蛋白α链和β链的人工片段。天然或合成的甘氨酸-赖氨酸-缬氨酸-天冬酰胺和苯丙氨酸-谷氨酸-组氨酸-谷氨酸具有一定的生长激素释放活性，而缬氨酸-色氨酸、酪氨酸-苯丙氨酸和赖氨酸-苯丙氨酸-酪氨酸具有轻微的催乳素释放活性。其他肽的生物活性尚未确定。
