Pawlowski A, Haberman H F, Menon I A
Cancer Res. 1980 Oct;40(10):3652-60.
A model for a metastasizing melanoma was developed, and its characteristics were established. Sixty-five albino guinea pigs were painted with 7,12-dimethylbenzanthracene in acetone. There was evidence that, after 18 months, 40% of the animals developed melanomas. Melanomas arose by a malignant transformation of junctional nevus cells and/or by transformation of amelanotic melanocytes. Metastases to the skin and internal organs were multiple and eventually fatal for the animals. Histology and electron microscopy of induced melanomas were reviewed in detail. Clinical and histological events leading to development of melanoma in albino guinea pigs were found to be similar to human melanomas in a number of aspects. Fragments of melanomas were successfully transplanted to "nude" mice and healthy albino guinea pigs. The described model could be used for study of the various cellular and tissue events which precede nevus, lentigo maligna, and melanoma formation. It could also be useful in studying carcinogenic potential, for studying development of metastases, and presumably for trials of treatment.
建立了一种转移性黑色素瘤模型，并确定了其特征。65只白化豚鼠用丙酮中的7,12-二甲基苯并蒽涂抹。有证据表明，18个月后，40%的动物发生了黑色素瘤。黑色素瘤由交界痣细胞的恶性转化和/或无色素黑素细胞的转化产生。皮肤和内脏器官的转移是多发性的，最终导致动物死亡。对诱导产生的黑色素瘤进行了详细的组织学和电子显微镜检查。发现白化豚鼠黑色素瘤发生过程中的临床和组织学事件在许多方面与人类黑色素瘤相似。黑色素瘤碎片成功移植到“裸”鼠和健康白化豚鼠体内。所描述的模型可用于研究痣、恶性雀斑和黑色素瘤形成之前的各种细胞和组织事件。它也可用于研究致癌潜力、转移的发展，大概还可用于治疗试验。
