Pawlowski A, Haberman H F, Menon I A
Cancer Res. 1976 Aug;36(8):2813-21.
Seventy albino guinea pigs were used in the experiment to investigate the influence of 9,10-dimethyl-1,2-benzanthracene on amelanotic melanocytes. A field 5 sq cm was marked on the flank of each animal. Hairs from these fields were clipped twice a week and painted for 20 consecutive weeks with 0.3 ml of 1% 9,10-dimethyl-1,2-benzanthracene in acetone. During the 1-year observation period, pigmented sports appeared in 40 animals. Biopsies were taken under local anesthesia, and sections were prepared for light and electron microscopic observations. Melanin formation of unknown mechanism took place in the epidermal melanocytes and in the melanocytes from the outer root sheath. These melanocytes also formed junctional and compound nevi; serial sections revealed various stages of pigmented nevi development. Schwann cells did not participate in the formation of nevi. Evidence is presented that 9,10-dimethyl-1,2-benzanthracene can convert amelanotic melanocytes into melanin-producing cells in the albino guinea pig skin. In addition, this system produces an animal model for the development of junctional and compound nevi.
70只白化豚鼠用于该实验，以研究9,10 - 二甲基 - 1,2 - 苯并蒽对无黑色素黑素细胞的影响。在每只动物的胁腹标记出一个5平方厘米的区域。每周两次剪掉该区域的毛发，并用0.3毫升1%的9,10 - 二甲基 - 1,2 - 苯并蒽丙酮溶液连续涂抹20周。在1年的观察期内，40只动物出现了色素沉着斑。在局部麻醉下进行活检，并制备切片用于光镜和电镜观察。表皮黑素细胞和外根鞘黑素细胞出现了机制不明的黑色素形成。这些黑素细胞还形成了交界痣和复合痣；连续切片显示了色素痣发展的各个阶段。雪旺细胞不参与痣的形成。有证据表明，9,10 - 二甲基 - 1,2 - 苯并蒽可使白化豚鼠皮肤中的无黑色素黑素细胞转变为产生黑色素的细胞。此外，该系统为交界痣和复合痣的发展提供了一个动物模型。
