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大肠杆菌核糖体蛋白L11与巨大芽孢杆菌蛋白BM-L11之间的功能同源性。

Functional homology between E. coli ribosomal protein L11 and B. megaterium protein BM-L11.

作者信息

Stark M J, Cundliffe E, Dijk J, Stöffler G

出版信息

Mol Gen Genet. 1980;180(1):11-5. doi: 10.1007/BF00267346.

DOI:10.1007/BF00267346
PMID:6777629
Abstract

Ribosomes from the thiostrepton-resistant mutant MJ1 of Bacillus megaterium completely lack a protein designated BM-L11. When assayed in vitro, such ribosomes show an impaired ability to hydrolyse GTP in the presence of the elongation factor EF-G and are unable to support the synthesis of (p)ppGpp in response to the stringent factor. Restoration of both these activities can be achieved by re-addition of either protein BM-L11 or its serological homologue from Escherichia coli, protein L11, implying that these two proteins are related functionally as well as immunologically.

摘要

巨大芽孢杆菌硫链丝菌素抗性突变体MJ1的核糖体完全缺乏一种名为BM-L11的蛋白质。在体外检测时，此类核糖体在延伸因子EF-G存在的情况下水解GTP的能力受损，并且在受到严谨因子作用时无法支持（p）ppGpp的合成。通过重新添加蛋白质BM-L11或其来自大肠杆菌的血清学同源物蛋白质L11，这两种活性均可恢复，这意味着这两种蛋白质在功能上以及免疫学上均相关。

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本文引用的文献

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Mutants of Escherichia coli lacking ribosomal protein L11.缺乏核糖体蛋白L11的大肠杆菌突变体。
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Thiostrepton-resistant mutants of Bacillus subtilis: localization of resistance to the 50S subunit.枯草芽孢杆菌硫链丝菌素抗性突变体：对50S亚基抗性的定位
Mol Gen Genet. 1976 Mar 30;144(3):235-41. doi: 10.1007/BF00341721.
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Purification of ribosomal proteins from Escherichia coli under nondenaturing conditions.在非变性条件下从大肠杆菌中纯化核糖体蛋白。
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Purification and properties of stringent factor.
J Biol Chem. 1975 Feb 25;250(4):1212-7.
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10
Ribosomes in thiostrepton-resistant mutants of Bacillus megaterium lacking a single 50 S subunit protein.巨大芽孢杆菌硫链丝菌素抗性突变体中的核糖体，缺少一种单一的50 S亚基蛋白。
J Mol Biol. 1979 Aug 5;132(2):235-52. doi: 10.1016/0022-2836(79)90393-0.