枯草芽孢杆菌硫链丝菌素抗性突变体：对50S亚基抗性的定位
Thiostrepton-resistant mutants of Bacillus subtilis: localization of resistance to the 50S subunit.
作者信息
Pestka S, Weiss D, Vince R, Wienen B, Stöffler G, Smith I
出版信息
Mol Gen Genet. 1976 Mar 30;144(3):235-41. doi: 10.1007/BF00341721.
A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes of S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and -resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.
获得了许多枯草芽孢杆菌硫链丝菌素抗性突变体。硫（thi）突变位于靠近strA的位置。硫链丝菌素对亲本菌株和突变菌株S-100组分核糖体合成聚苯丙氨酸的影响表明，抗性定位于核糖体。此外，硫链丝菌素对硫链丝菌素敏感和抗性菌株核糖体及50S亚基结合[3H]GTP的影响将抗性位点定位于50S亚基。另外，还获得了从硫链丝菌素抗性回复到硫链丝菌素敏感性的回复突变体。这些硫链丝菌素敏感回复突变体的核糖体和50S亚基对硫链丝菌素敏感，类似于亲本敏感的枯草芽孢杆菌。
Zotero 插件