Shukla P T, Auerbach C
Mutat Res. 1980 Sep;72(2):231-43. doi: 10.1016/0027-5107(80)90037-8.
We have developed genetic tests for estimating the proportion of small deletions among chemically induced point mutations in Drosophila. The criteria used allow the detection of deletions that are large enough to include a viable visible mutation as well as a lethal, or a sex-linked lethal as well as a gene that is required for the development of a spermatogonium into a spermatozoon. On these criteria, we have concluded that DEB produces a high proportion of deletions among point mutations; that HA produces no deletions; and that DEN produces either no deletions or only very small ones that cannot be detected by our methods.
我们已经开发出了基因检测方法，用于估算果蝇化学诱导点突变中小缺失的比例。所采用的标准能够检测出足够大的缺失，这些缺失包含一个可行的可见突变以及一个致死突变，或者一个性连锁致死突变以及精原细胞发育成精子所需的一个基因。基于这些标准，我们得出结论：二乙基亚硝胺（DEB）在点突变中产生高比例的缺失；六氨基嘌呤（HA）不产生缺失；而1,2-二甲基肼（DEN）要么不产生缺失，要么只产生我们的方法无法检测到的非常小的缺失。
