DNA长度对核小体低盐转变的影响。
Effect of DNA length on the nucleosome low salt transition.
作者信息
Dieterich A E, Eshaghpour H, Crothers D M, Cantor C R
出版信息
Nucleic Acids Res. 1980 Jun 11;8(11):2475-87. doi: 10.1093/nar/8.11.2475.
The effect of DNA length on the low salt unfolding transition of nucleosomes has been studied by the use of fluorescently labeled histones. Nucleosomes were formed by the reconstitution of bulk DNA fragments averaging 173 and 250 base pairs in length. These nucleosomes exhibited a conformational change in a transition centered at about 7 mM ionic strength, very different from that observed for the standard 145 bp nucleosomes (1-3mM). In addition, the conformational change of the 173 and 250 bp nucleosomes involves twice as many ions as that of the 145 bp nucleosomes.
通过使用荧光标记的组蛋白，研究了DNA长度对核小体低盐展开转变的影响。通过重组平均长度为173和250个碱基对的大量DNA片段形成核小体。这些核小体在以约7 mM离子强度为中心的转变中表现出构象变化，这与标准145 bp核小体（1-3 mM）观察到的情况非常不同。此外，173和250 bp核小体的构象变化涉及的离子数量是145 bp核小体的两倍。
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