Skogen B, Natvig J B, Bøorresen A L, Berg K
Scand J Immunol. 1980;11(6):643-8. doi: 10.1111/j.1365-3083.1980.tb00033.x.
After incubation of protein SAA-containing rabbit serum at 37 degrees C overnight, the strength of the precipitation reaction against antiserum to SAA was decreased. This was at east partly due to enzymatic degradation of protein SAA. The enzymatic activity was not strongly associated with the SAA-high-density lipoprotein (HDL) complex, since it sedimented in the preparative ultracentrifuge at a density at which the SAA-HDL complex floats. The degrading component was obtained in concentrated form from rabbit and human sera by adsorption to Sepharose 4B. Degradation of human SAA by the human serum component was inhibited with disopropyl fluorophosphate, an inhibitor of serine proteases.
含蛋白质SAA的兔血清在37℃孵育过夜后，针对SAA抗血清的沉淀反应强度降低。这至少部分是由于蛋白质SAA的酶促降解。该酶活性与SAA-高密度脂蛋白（HDL）复合物的关联不强，因为它在制备型超速离心机中以SAA-HDL复合物漂浮的密度沉淀。通过吸附到琼脂糖4B上，从兔血清和人血清中以浓缩形式获得降解成分。人血清成分对人SAA的降解被丝氨酸蛋白酶抑制剂二异丙基氟磷酸抑制。
