Anniko M, Werner S, Wersäll J
Acta Otolaryngol. 1980;90(1-2):143-51. doi: 10.3109/00016488009131710.
Human pituitary adenomas, when preserved as organ cultures do not lose their function of synthesizing and releasing hormone. Thus, growth hormone producing adenomas have been cultured for 35 days and prolactinomas for 14 days with maintained hormone hypersecretion, > 30 000 pmol/l/48 h of growith hormone and > 15 000 micrograms/l/48 h of prolactin. Preserved morphology/ultrastructure was documented in these specimens. Following repeated renewal of the entire nutrient solution, less than 1/10 of the initial hormone content remained after the first change, indicating an active hormone production/release into the culture medium as hormone concentration rose to previous levels. The growth hormone producing cells could be stimulated by TRH and LH-RH in the concentrations 10(-8) and 10(-7) g/l respectively, indicating the same pathological reactivity in vitro as in vivo. Prolactin synthesis in vitro paralleled that in vivo, as reflected by serum prolactin levels. Growth hormone synthesis in vitro paralleled the clinical activity of the disease, but the plasma hormone levels in patients with acromegaly did not do so.
人垂体腺瘤在作为器官培养物保存时不会丧失其合成和释放激素的功能。因此，分泌生长激素的腺瘤已培养35天，催乳素瘤已培养14天，激素分泌仍处于亢进状态，生长激素超过30000 pmol/l/48小时，催乳素超过15000微克/l/48小时。这些标本的形态/超微结构得以保留。在整个营养液反复更新后，第一次更换后剩余的激素含量不到初始含量的1/10，这表明随着激素浓度回升至先前水平，有活跃的激素产生并释放到培养基中。分泌生长激素的细胞可分别被浓度为10(-8)和10(-7) g/l的促甲状腺激素释放激素（TRH）和促黄体生成素释放激素（LH-RH）刺激，这表明其在体外与体内具有相同的病理反应性。体外催乳素的合成与体内情况相似，这可通过血清催乳素水平反映出来。体外生长激素的合成与疾病的临床活动情况相似，但肢端肥大症患者的血浆激素水平并非如此。
