Wasserman L, Shlesinger H, Abramovici A, Goldman J A, Allalouf D
Am J Obstet Gynecol. 1980 Dec 1;138(7 Pt 1):769-73. doi: 10.1016/s0002-9378(16)32734-x.
Crude glycosaminoglycans were prepared from acetone powder of diabetic, toxemic, and normal term placentas. Glycosaminoglycan composition was determined by electrophoresis and densitometric scanning with and without treatment with testicular hyaluronidase and chondroitinase ABC. The identity of individual glycosaminoglycans was confirmed by the nature of their hexosamine. Glycosaminoglycan content was found to be significantly increased in diabetic placentas and increased to a lesser degree in the toxemic placentas. The amount of hyaluronic acid was elevated in both abnormal tissues, and heparan sulfate was slightly higher in diabetes, while unchanged in toxemia. Dermatan sulfate was markedly reduced in the abnormal placentas while chondroitin 4/6 sulfate was unaltered. An attempt was made to correlate the histopathologic changes reported to occur in these conditions with the alterations in the glycosaminoglycans patterns of placentas.
从糖尿病、毒血症和足月正常胎盘的丙酮粉中制备粗糖胺聚糖。通过电泳和光密度扫描测定糖胺聚糖的组成，分别在有和没有用睾丸透明质酸酶和软骨素酶ABC处理的情况下进行。通过其己糖胺的性质确认了各个糖胺聚糖的身份。发现糖尿病胎盘的糖胺聚糖含量显著增加，毒血症胎盘的糖胺聚糖含量增加程度较小。两种异常组织中的透明质酸含量均升高，糖尿病患者的硫酸乙酰肝素含量略高，而毒血症患者的硫酸乙酰肝素含量未变。异常胎盘中的硫酸皮肤素明显减少，而硫酸软骨素4/6未改变。试图将据报道在这些情况下发生的组织病理学变化与胎盘糖胺聚糖模式的改变联系起来。
