Phillips W E, Kaufman L
Am J Vet Res. 1980 Aug;41(8):1263-5.
Sixteen sera from 17 dogs with blastomycosis produced a precipitin band identical with the diagnostically significant precipitin A band formed by a Blastomyces dermatitidis reference antiserum and a soluble B dermatitidis yeast-form antigen in the agar-gel immunodiffusion test (94% sensitivity). The other serum from a dog with histopathologic demonstration of B dermatitidis in pulmonic tissues produced an unrelated precipitin band. Sixteen of the 17 diagnoses made by the detection of precipitin A were confirmed by isolation and culture of B dermatitidis or by histopathologic demonstration of the pathogen. Three cases were confirmed by cultural isolation only, 10 by histopathologic demonstration only, and 3 by both. In three other dogs given amphotericin B, there were demonstrable changes in serum precipitin A reactions.
从17只患有芽生菌病的犬身上采集的16份血清，在琼脂凝胶免疫扩散试验中产生了一条沉淀带，该沉淀带与皮炎芽生菌参考抗血清和可溶性皮炎芽生菌酵母型抗原形成的具有诊断意义的沉淀素A带相同（敏感性为94%）。另一只在肺部组织经组织病理学证实患有皮炎芽生菌的犬的血清产生了一条不相关的沉淀带。通过检测沉淀素A做出的17例诊断中，有16例通过皮炎芽生菌的分离培养或病原体的组织病理学证实得到了确认。3例仅通过培养分离得到确认，10例仅通过组织病理学证实得到确认，3例通过两者均得到确认。在另外3只接受两性霉素B治疗的犬中，血清沉淀素A反应出现了明显变化。
