Khansari N, Segre D, Segre M
Am J Vet Res. 1982 Dec;43(12):2279-83.
An antiglobulin hemagglutination test was developed for detection of antibody directed to Histoplasma capsulatum and Blastomyces dermatitidis. The substances responsible for spontaneous agglutination of erythrocytes were removed from histoplasmin and blastomycin by vacuum dialysis, and partial purification of the antigens by gel filtration chromatography on Sephadex G-150 allowed removal of additional nonantigenic material which competed with the antigens for binding on the erythrocyte surface. The test was sensitive enough to detect antibodies in sera which were negative by complement fixation, immunodiffusion, or both, but it failed to discriminate reliably between antibody directed to H capsulatum and antibody directed to B dermatitidis. Erythrocytes sensitized with partially purified blastomycin produced some false-positive reactions with normal canine sera; this was corrected by diluting the antigen before sensitization of the erythrocytes.
开发了一种抗球蛋白血凝试验，用于检测针对荚膜组织胞浆菌和皮炎芽生菌的抗体。通过真空透析从组织胞浆菌素和芽生菌素中去除导致红细胞自发凝集的物质，并且通过在葡聚糖凝胶G - 150上进行凝胶过滤色谱法对抗原进行部分纯化，从而去除了与抗原竞争结合红细胞表面的其他非抗原性物质。该试验灵敏度足以检测补体结合试验、免疫扩散试验或两者均为阴性的血清中的抗体，但未能可靠地区分针对荚膜组织胞浆菌的抗体和针对皮炎芽生菌的抗体。用部分纯化的芽生菌素致敏的红细胞与正常犬血清产生了一些假阳性反应；通过在红细胞致敏前稀释抗原对其进行了校正。
