Endogenous LH, FSH, TSH, growth hormone, prolactin and insulin were measured by radioimmunoassay in human plasma samples stored at 4 degrees, 20 degrees and 37 degrees for up to 8 days or repeatedly frozen and thawed. 2. At 4 degrees, the concentrations of all hormones were stable for at least 8 days; at 20 degrees only LH, FSH and TSH were stable for 8 days; at 37 degrees only TSH was stable for 8 days. 3. All the hormones except insulin were stable during 5 freeze-thaw cycles.