Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Faculty of Laboratory Medicine, Center for Translational Medicine, North Sichuan Medical college, Nanchong, China.
J Clin Lab Anal. 2020 Jul;34(7):e23255. doi: 10.1002/jcla.23255. Epub 2020 Mar 4.
To investigate the influence of storage time and temperature on plasma insulin levels and to establish a correction formula.
Venous blood samples were taken from 20 volunteers and processed as follows: whole blood samples, centrifuged samples, and separated plasma samples were stored at 4°C or 25°C. Insulin levels were determined by direct chemiluminescence at 0, 0.5, 1, 2, 4, and 8 hours. According to the correlation between the insulin concentration ratio and storage time, correction formulas for the insulin concentration were established. To verify the test, the venous blood samples of another 33 volunteers were processed in the same way. The insulin levels of the samples were corrected after 3, 6, 12, and 24 hours and compared with the value at 0 hours to verify the feasibility of the corrected formula.
With the prolongation of storage time, the insulin levels of the whole blood samples at 4°C or 25°C and of the centrifuged samples at 25°C decreased gradually (P < .001), and the insulin level correction formulas were C = C /0.991e , C = C /1.048e , and C = C /[-0.068ln(x) + 0.9242]. There was no significant difference between the corrected insulin results and the original results at any time within 12 hours (P > .05).
The insulin levels of the whole blood samples at 4°C or 25°C and of the plasma samples at 25°C gradually decreased with storage time. The effect of storage time on the insulin level can be reduced with the correction formulas.
探讨储存时间和温度对血浆胰岛素水平的影响，并建立校正公式。
采集 20 名志愿者的静脉血样，分别进行全血样本、离心样本和分离血浆样本处理，4°C 或 25°C 储存。在 0、0.5、1、2、4 和 8 小时时通过直接化学发光法测定胰岛素水平。根据胰岛素浓度比值与储存时间的相关性，建立胰岛素浓度校正公式。为了验证该检验，对另外 33 名志愿者的静脉血样以相同方式进行处理。在 3、6、12 和 24 小时后对样本的胰岛素水平进行校正，并与 0 小时的数值进行比较，以验证校正公式的可行性。
随着储存时间的延长，4°C 或 25°C 全血样本和 25°C 离心样本的胰岛素水平逐渐下降（P<.001），胰岛素水平校正公式为 C=C/0.991e、C=C/1.048e 和 C=C/[-0.068ln(x)+0.9242]。在 12 小时内的任何时间，校正后胰岛素结果与原始结果均无显著差异（P>.05）。
4°C 或 25°C 全血样本和 25°C 血浆样本的胰岛素水平随储存时间逐渐下降。通过校正公式可以降低储存时间对胰岛素水平的影响。
