Goodman H M, Kostyo J L
Endocrinology. 1981 Feb;108(2):553-8. doi: 10.1210/endo-108-2-553.
A highly purified preparation of human GH (hGH) and two large fragment complexes (Da1 and Dc2) isolated from plasmin digests of reduced and S-carbamidomethylated hGH were tested for their ability to produce five different biological responses in segments of epididymal fat obtained from hypophysectomized rats. Judging by the minimal concentration of hormone needed to produce a statistically significant response, hGH was 3-10 times as potent as Da1 and Dc2 in increasing the oxidation of [U-14C]glucose to 14CO2, hGH and Da1 were equipotent in stimulating the oxidation of L-[1-14C]leucine to 14CO2, antagonizing the lipolytic effect of epinephrine, and inducing refractoriness to the insulin-like action of hGH. At least 3 times as much Dc2 was required to produce significant responses. Da1 was 10 times more active than hGH in producing delayed lipolysis in the presence of theophylline and 30 times as effective as Dc2. These findings suggest that the various responses to GH that can be measured in adipose tissue may result from more than one kind of interaction between GH and adipocytes. (Endocrinology 108: 553, 1981)
对一种高度纯化的人生长激素（hGH）制剂以及从还原型和S-氨甲酰甲基化hGH的纤溶酶消化物中分离出的两种大片段复合物（Da1和Dc2），检测了它们在取自垂体切除大鼠的附睾脂肪片段中产生五种不同生物学反应的能力。根据产生统计学显著反应所需的最低激素浓度判断，在将[U-¹⁴C]葡萄糖氧化为¹⁴CO₂方面，hGH的效力是Da1和Dc2的3至10倍；在刺激L-[¹-¹⁴C]亮氨酸氧化为¹⁴CO₂、拮抗肾上腺素的脂解作用以及诱导对hGH胰岛素样作用的不应性方面，hGH和Da1效力相当。产生显著反应至少需要3倍量的Dc2。在茶碱存在的情况下，Da1在产生延迟脂解方面比hGH活跃10倍，比Dc2有效30倍。这些发现表明，在脂肪组织中可检测到的对GH的各种反应可能源于GH与脂肪细胞之间不止一种类型的相互作用。（《内分泌学》108: 553, 1981）
