Bibel D J, Lovell D J, Smiljanic R J
Appl Environ Microbiol. 1978 Jun;35(6):1128-35. doi: 10.1128/aem.35.6.1128-1135.1978.
The colonization and survival of Bacillus species, members of the cutaneous microbial community of humans, were investigated by applying spores of Bacillus licheniformis to the forearms of volunteers. Four strains were tested, including the bacitracin producer ATCC 10716 and its bacitracin-negative mutant. Germination occurred within 24 h. Significant differences in survival population and duration were found among the test strains; however, ATCC 10716 and its mutant produced statistically similar survival curves. In general, an inoculum density of 10(4) colony-forming units per cm2 allowed survival for at least 2 weeks. Individual variation was extreme, for one subject harbored bacilli for over 2 months and another eliminated the microorganism within 3 days. Individuals could be differentiated into long-term (greater than 21 days) and short-term (less than 14 days) carriers. Eight of the 11 volunteers (73%) inoculated with ATCC 10716 carried it for 2 weeks, and 5 subjects (45%) continued to support the bacilli for 3 weeks. Spreading of the organism to other regions of the body occurred, but bacilli were not detected in these areas beyond 6 days.
通过将地衣芽孢杆菌的孢子涂抹在志愿者的前臂上，对作为人类皮肤微生物群落成员的芽孢杆菌属的定殖和存活情况进行了研究。测试了四株菌株，包括杆菌肽产生菌ATCC 10716及其杆菌肽阴性突变体。孢子在24小时内萌发。各测试菌株在存活数量和持续时间上存在显著差异；然而，ATCC 10716及其突变体产生的存活曲线在统计学上相似。一般来说，每平方厘米10(4)个菌落形成单位的接种密度可使细菌存活至少2周。个体差异极大，一名受试者携带芽孢杆菌超过2个月，而另一名受试者在3天内就清除了这种微生物。个体可分为长期（超过21天）和短期（少于14天）携带者。接种ATCC 10716的11名志愿者中有8名（73%）携带该菌2周，5名受试者（45%）在3周内仍携带该芽孢杆菌。该微生物会扩散到身体的其他部位，但在6天后这些部位未检测到芽孢杆菌。
