Liebich H M, Pickert A, Stierle U, Wöll J
J Chromatogr. 1980 Oct 31;199:181-9. doi: 10.1016/s0021-9673(01)91371-8.
Saturated and unsaturated dicarboxylic acids in urine are analyzed within the total profile of organic acids, using the methyl ester derivatives. Twenty-three acids with two carboxyl groups were identified. The method is employed for comparative studies of the excretion of dicarboxylic acids by individuals with normal and with increased fatty acid oxidation. In the group of the unsaturated acids, the cis-trans isomers mesaconic acid and citraconic acid, the two isomers of 3-methylglutaconic acid and muconic acid are characterized by the mass spectra of their methyl esters. The saturated unbranched and even-numbered dicarboxylic acids are elevated during fasting and diabetic ketoacidosis. In the total profile of the organic acids, succinic and adipic acid are indicators for ketoacidotic states.
尿液中的饱和与不饱和二元羧酸在有机酸总谱中进行分析，采用甲酯衍生物。共鉴定出23种含两个羧基的酸。该方法用于比较脂肪酸氧化正常和增加的个体中二元羧酸的排泄情况。在不饱和酸组中，顺反异构体中康酸和柠康酸、3-甲基戊二酸和粘康酸的两种异构体通过其甲酯的质谱进行表征。饱和无支链且偶数碳的二元羧酸在禁食和糖尿病酮症酸中毒时升高。在有机酸总谱中，琥珀酸和己二酸是酮症酸中毒状态的指标。
