Zubrzycki L, Weinberger S S
Sex Transm Dis. 1980 Oct-Dec;7(4):183-7. doi: 10.1097/00007435-198010000-00008.
A temperature-sensitive mutant of Neisseria gonorrhoeae strain tsA-1 was used in a transformation test for the laboratory diagnosis of gonorrhea. This transformation test (C test) is based on a spotting of a DNA lysate, obtained through simple base-acid extraction of a cervical-swab specimen, onto a lawn of tsA-1, which is then incubated for one to two days at 37 C. Of 1,053 cervical-swab specimens tested, 52 contained the gonococcal DNA necessary for restoring the ability of tsA-1 to grow well at 37 C; 49 of the 52 specimens were identified as N. gonorrhoeae by routine diagnostic laboratory procedures. The time required for the C test is two to three days, which is shorter than that required for routine diagnostic laboratory tests, and the C test involves a minimum of common laboratory supplies and expertise. The discrepancies between results of the C test and routine procedures are explained as follows. In one case an isolate suspected to be N. gonorrhoeae was nonreactive in the sugar fermentation test, and in two cases the agar plates were overgrown with yeasts; neither situation affected the C test.
淋病奈瑟菌菌株tsA - 1的温度敏感突变体被用于淋病实验室诊断的转化试验。这种转化试验（C试验）基于将通过宫颈拭子标本简单酸碱提取获得的DNA裂解物点样到tsA - 1菌苔上，然后在37℃下孵育一到两天。在检测的1053份宫颈拭子标本中，52份含有使tsA - 1在37℃下恢复良好生长能力所需的淋球菌DNA；通过常规诊断实验室程序，这52份标本中的49份被鉴定为淋病奈瑟菌。C试验所需时间为两到三天，比常规诊断实验室试验所需时间短，并且C试验所需的常见实验室耗材和专业知识最少。C试验结果与常规程序结果之间的差异解释如下。在一个案例中，一株疑似淋病奈瑟菌的分离株在糖发酵试验中无反应，在两个案例中，琼脂平板上长满了酵母菌；这两种情况都不影响C试验。
