Bailey G S, Nixon J E, Hendricks J D, Sinnhuber R O, Van Holde K E
Biochemistry. 1980 Dec 9;19(25):5836-42. doi: 10.1021/bi00566a027.
The purpose of this work was to investigate the distribution in chromatin of deoxyribonucleic acid (DNA) adducts of aflatoxin B1, following exposure in vivo. Rainbow trout were injected intraperitoneally with radiolabeled aflatoxin B1, a potent procarcinogen known to readily induced hepatocellular carcinomas in these fish. After maximum incorporation, liver nuclei were prepared and digested with micrococcal nuclease. Mono-, di-, and trinucleosomal fractions were purified from several stages of nuclease digestion, and the lengths and specific activities of their DNA were determined. The results indicate that aflatoxin B1 is approximately 5 times as likely on a per nucleotide basis to localize on internucleosomal (linker) DNA as on nucleosomal core DNA in this system.
这项工作的目的是研究黄曲霉毒素B1的脱氧核糖核酸（DNA）加合物在体内暴露后在染色质中的分布情况。虹鳟鱼经腹腔注射放射性标记的黄曲霉毒素B1，这是一种已知能在这些鱼中轻易诱发肝细胞癌的强效前致癌物。在最大程度掺入后，制备肝细胞核并用微球菌核酸酶消化。从核酸酶消化的几个阶段中纯化出单核小体、双核小体和三核小体组分，并测定其DNA的长度和比活性。结果表明，在该系统中，基于每个核苷酸计算，黄曲霉毒素B1定位在核小体间（连接区）DNA上的可能性约为定位在核小体核心DNA上的5倍。
