Laborde N P, Wolfsen A R, Odell W D
Endocrinology. 1981 Jan;108(1):72-5. doi: 10.1210/endo-108-1-72.
Studies were designed to assess whether a short loop feedback control for FSH existed in the rabbit. Castrated adult female animals bearing chronically implanted Silastic catheters to permit frequent blood sampling were studied without anesthesia. Ovine FSH was administered as an iv bolus in doses ranging between 0.1-500 micrograms. Endogenous rabbit FSH was quantified using a RIA that did not cross-react with ovine FSH. Blood samples were obtained before and 5, 10, 15, 30, 60, 120, 180, 240, and 300 min after the injection. Each animal was tested at two or more dose levels on different days. Ovine FSH produced suppression of rabbit FSH secretion within 5 min after injection. The minimum effective dose was 1 microgram; maximal suppression occurred with 50-100 micrograms ovine FSH. This short loop feedback control system was specific for FSH; ovine FSH, even at high doses, failed to suppress endogenous rabbit LH. This is the first direct demonstration of a negative short loop feedback control for FSH and the first entirely specific control for the FSH system to be described.
开展研究以评估家兔体内是否存在针对促卵泡激素（FSH）的短反馈回路控制。对成年去势雌性动物进行研究，这些动物长期植入硅橡胶导管以允许频繁采血，且未实施麻醉。以静脉推注的方式给予绵羊促卵泡激素（oFSH），剂量范围为0.1至500微克。使用与绵羊促卵泡激素无交叉反应的放射免疫分析法（RIA）对家兔内源性促卵泡激素进行定量分析。在注射前以及注射后5、10、15、30、60、120、180、240和300分钟采集血样。每只动物在不同日期接受两个或更多剂量水平的测试。绵羊促卵泡激素在注射后5分钟内即可抑制家兔促卵泡激素的分泌。最小有效剂量为1微克；50至100微克绵羊促卵泡激素可产生最大抑制作用。这种短反馈回路控制系统对促卵泡激素具有特异性；即使高剂量的绵羊促卵泡激素也无法抑制家兔内源性促黄体生成素（LH）。这是首次直接证明存在针对促卵泡激素的负性短反馈回路控制，也是首次描述的完全针对促卵泡激素系统的特异性控制。
