Patritti-Laborde N, Odell W D
Fertil Steril. 1978 Oct;30(4):456-60.
The short-loop feedback control of rabbit luteinizing hormone (rLH) was studied by using a highly specific radioimmunoassay (RIA) system for rLH which does not react with human LH. Permanent intravenous catheters were placed in adult female New Zealand White rabbits at the time of castration. Highly purified human luteinizing hormone (hLH) was injected intravenously at doses of 0.1, 0.3, 0.5, 1, 2, 3, 4, 10, 50, and 100 IU into unanesthetized animals 1 to 16 days following castration. Blood samples were obtained at -20, 0, 5, 10, 20, 30, 60, 120, and 180 minutes via catheter, and rLH and rabbit follicle-stimulating hormone (rFSH) levels were determined by RIA. Doses between 1 and 100 IU of hLH produced a prompt decrease in rLH (within 5 minutes); the maximal response occurred within 20 to 30 minutes. Calculated as integrated area of response between 0 and 180 minutes, a dose-response relationship existed between 0.5 and 4.0 IU of hLH. Human LH, even at high doses of 50 IU, produced no changes in endogeneous rFSH. This is the first description of an entirely specific control system for LH, separate from FSH. The short-loop feedback control system for LH is sensitive to levels of LH estimated to be present in eugonadal animals.
利用一种对兔促黄体激素（rLH）具有高度特异性且不与人促黄体激素发生反应的放射免疫分析（RIA）系统，对兔促黄体激素的短反馈回路控制进行了研究。在成年雌性新西兰白兔阉割时，植入永久性静脉导管。在阉割后1至16天，将高纯度人促黄体激素（hLH）以0.1、0.3、0.5、1、2、3、4、10、50和100国际单位的剂量静脉注射到未麻醉的动物体内。通过导管在-20、0、5、10、20、30、60、120和180分钟采集血样，并用放射免疫分析法测定rLH和兔促卵泡激素（rFSH）水平。1至100国际单位的hLH剂量可使rLH迅速降低（5分钟内）；最大反应在20至30分钟内出现。以0至180分钟的反应积分面积计算，hLH在0.5至4.0国际单位之间存在剂量反应关系。即使是50国际单位的高剂量人促黄体激素，也不会使内源性rFSH发生变化。这是首次对与促卵泡激素分开的、完全特异性的促黄体激素控制系统进行描述。促黄体激素的短反馈回路控制系统对正常性腺动物中估计存在的促黄体激素水平敏感。
