National Institute of Agrobiological Resources, Tsukuba Science City, 305, Yatabe Ibaraki, Japan.
Plant Mol Biol. 1985 Nov;4(6):355-64. doi: 10.1007/BF02418257.
The nucleotide sequence of a spacer region between rice 17S and 25S rRNA genes (rDNAs) has been determined. The coding regions for the mature 17S, 5.8S and 25S rRNAs were identified by sequencing terminal regions of these rRNAs. The first internal transcribed spacer (ITS1), between 17S and 5.8S rDNAs, is 194-195 bp long. The second internal transcribed spacer (ITS2), between 5.8S and 25S rDNAs, is 233 bp long. Both spacers are very rich in G+C, 72.7% for ITS1 and 77.3% for ITS2. The 5.8S rDNA is 163-164 bp long and similar in primary and secondary structures to other eukaryotic 5.8S rDNAs. The 5.8S rDNA is capable of interacting with the 5' terminal region of 25S rDNA.
已测定了水稻 17S 和 25S rRNA 基因(rDNA)之间间隔区的核苷酸序列。通过对这些 rRNA 的末端区域进行测序,鉴定出成熟的 17S、5.8S 和 25S rRNA 的编码区。17S 和 5.8S rDNA 之间的第一个内部转录间隔区(ITS1)长 194-195bp。5.8S 和 25S rDNA 之间的第二个内部转录间隔区(ITS2)长 233bp。两个间隔区富含 G+C,ITS1 为 72.7%,ITS2 为 77.3%。5.8S rDNA 长 163-164bp,在一级和二级结构上与其他真核生物 5.8S rDNA 相似。5.8S rDNA 能够与 25S rDNA 的 5'末端区域相互作用。