Jobe A, Ikegami M, Glatz T, Yoshida Y, Diakomanolis E, Padbury J
J Clin Invest. 1981 Feb;67(2):370-5. doi: 10.1172/JCI110044.
Premature lambs were treated with 50 mg/kg of natural surfactant lipid by tracheal instillation either at birth or shortly thereafter when respiratory failure was documented. All lambs were delivered by cesarean section and supported on infant ventilators with 100% oxygen under conditions to mimic the care of human infants with the respiratory distress syndrome. The natural surfactant used for therapy was recovered by lavage from sheep lung. Six 120-d gestational age lambs treated at birth had an initial mean oxygen pressure (pO2) value of 270 +/- 35 mm Hg; this fell within 3 h to less than 100 mm Hg. By 8.3 +/- 0.3 h after birth the lambs were in severe respiratory failure with a mean pH less than 7.1 and a mean pCO2 greater than 70 mm Hg. Six untreated lambs had pH values below 7.0 within 40 min of life despite more intensive respiratory support than was given the treated animals. Treatment with natural surfactant of 17 lambs of 120 and 130 d gestational age after early respiratory failure resulted in a prompt increase in pO2 values from about 35 mm Hg to values over 200 mm Hg and a fall in pCO2 values to normal levels in the majority of animals. This response lasted only approximately 3 h, and a second treatment was less predictably effective.
早产羔羊在出生时或出生后不久出现呼吸衰竭时，通过气管内滴注50毫克/千克的天然表面活性脂质进行治疗。所有羔羊均通过剖宫产分娩，并在模拟患有呼吸窘迫综合征的人类婴儿护理条件下，使用婴儿呼吸机给予100%氧气支持。用于治疗的天然表面活性剂是通过从羊肺灌洗回收的。六只出生时接受治疗的妊娠120天龄羔羊的初始平均氧分压（pO2）值为270±35毫米汞柱；该值在3小时内降至100毫米汞柱以下。出生后8.3±0.3小时，羔羊出现严重呼吸衰竭，平均pH值低于7.1，平均pCO2大于70毫米汞柱。六只未治疗的羔羊在出生后40分钟内pH值低于7.0，尽管给予了比治疗动物更强的呼吸支持。对17只妊娠120天和130天龄且出现早期呼吸衰竭的羔羊使用天然表面活性剂治疗后，大多数动物的pO2值迅速从约35毫米汞柱升至200毫米汞柱以上，pCO2值降至正常水平。这种反应仅持续约3小时，第二次治疗的效果则较难预测。
