Denny P A, Denny P C, Jenkins K
Carbohydr Res. 1980 Dec 15;87(2):265-74. doi: 10.1016/s0008-6215(00)85213-1.
A sialomucin from the mouse submandibular gland was isolated and purified by a protocol involving Sephacryl S-200 chromatography, acidic dialysis, and preparative, poly(acrylamide)-gel electrophoresis. The mucus glycoprotein was judged to be free from contaminants by analytical and sodium dodecyl sulfate-poly(acrylamide)-gel electrophoresis, isoelectric focusing, immunoelectrophoresis, and immunodiffusion when made visible by Stains-all, periodic acid-Schiff reagent, and Coomassie Blue. The carbohydrate portion constituted 81% of the weight of the mucus glycoprotein, and was composed of 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, sialic acid, D-galactose, and D-mannose. Neither L-fucose nor sulfate was detected. The aliphatic amino acids constituted 60% of the protein core. The sialomucin has an apparent mol. wt. of 140,000 by sodium dodecyl sulfate-gel electrophoresis, and a pI of 2.77-3.63 by isoelectric focusing.
通过包括Sephacryl S - 200层析、酸性透析和制备性聚丙烯酰胺凝胶电泳的方案，从小鼠下颌下腺分离并纯化了一种唾液酸糖蛋白。通过分析型和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳、等电聚焦、免疫电泳和免疫扩散，并用全染剂、高碘酸 - 席夫试剂和考马斯亮蓝显色后，判断该黏液糖蛋白不含污染物。碳水化合物部分占黏液糖蛋白重量的81%，由2 - 乙酰氨基 - 2 - 脱氧 - D - 葡萄糖、2 - 乙酰氨基 - 2 - 脱氧 - D - 半乳糖、唾液酸、D - 半乳糖和D - 甘露糖组成。未检测到L - 岩藻糖和硫酸盐。脂肪族氨基酸占蛋白质核心的60%。通过十二烷基硫酸钠凝胶电泳，该唾液酸糖蛋白的表观分子量为140,000，通过等电聚焦，其pI为2.77 - 3.63。
