苏氨酸作为大肠杆菌的碳源。
Threonine as a carbon source for Escherichia coli.
作者信息
Chan T T, Newman E B
出版信息
J Bacteriol. 1981 Mar;145(3):1150-3. doi: 10.1128/jb.145.3.1150-1153.1981.
Abstract
Threonine can be used aerobically as the sole source of carbon and energy by mutants of Escherichia coli K-12. The pathway used involves the conversion of threonine via threonine dehydrogenase to aminoketobutyric acid, which is further metabolized by aminoketobutyric acid ligase, forming acetyl coenzyme A and glycine. A strain devoid of serine transhydroxymethylase uses this pathway and excretes glycine as a waste product. Aminoketobutyric acid ligase activity was demonstrated after passage of crude extracts through Sephadex G100.
摘要
苏氨酸可被大肠杆菌K-12突变体作为唯一的碳源和能源进行需氧利用。所使用的途径包括通过苏氨酸脱氢酶将苏氨酸转化为氨基酮丁酸，氨基酮丁酸再由氨基酮丁酸连接酶进一步代谢，形成乙酰辅酶A和甘氨酸。缺乏丝氨酸转羟甲基酶的菌株利用该途径并将甘氨酸作为废物排出。粗提物通过葡聚糖G100柱后，显示出氨基酮丁酸连接酶活性。
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本文引用的文献
1
Tissue sulfhydryl groups.
Arch Biochem Biophys. 1959 May;82(1):70-7. doi: 10.1016/0003-9861(59)90090-6.
2
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Biochem J. 1969 May;112(5):657-71. doi: 10.1042/bj1120657.
3
Utilization of L-threnonine by a pseudomonad: a catabolic role for L-threonine aldolase.
Biochem J. 1969 Nov;115(3):603-5. doi: 10.1042/bj1150603.
4
Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases.
J Bacteriol. 1970 Apr;102(1):193-203. doi: 10.1128/jb.102.1.193-203.1970.
5
Derivation of glycine from threonine in Escherichia coli K-12 mutants.
J Bacteriol. 1975 Jun;122(3):810-7. doi: 10.1128/jb.122.3.810-817.1975.
6
Bacterial catabolism of threonine. Threonine degradation initiated by L-threonine acetaldehyde-lyase (aldolase) in species of Pseudomonas.
Biochem J. 1977 Aug 15;166(2):209-16. doi: 10.1042/bj1660209.
7
The use of glycine as nitrogen source by Escherichia coli K12.
Biochim Biophys Acta. 1976 Jan 14;421(1):97-105. doi: 10.1016/0304-4165(76)90173-2.
8
Hydroxy amino acid metabolism in Pseudomonas cepacia: role of L-serine deaminase in dissimilation of serine, glycine, and threonine.
J Bacteriol. 1979 Oct;140(1):240-5. doi: 10.1128/jb.140.1.240-245.1979.
9
Threonine deaminase from Salmonella typhimurium. Substrate-specific patterns of inhibition in an activator site-deficient form of the enzyme.
J Biol Chem. 1979 Feb 25;254(4):1074-9.
10
Role of threonine dehydrogenase in Escherichia coli threonine degradation.
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