Clark H B, Hartman B K, Raichle M E, Preskorn S H, Larson K B
Brain Res. 1981 Mar 16;208(2):311-23. doi: 10.1016/0006-8993(81)90560-6.
A small volume (5 microliters) common carotid arterial injection method is described for the quantitation of cerebral vascular extraction fractions (Et) of diffusion limited tracer molecules in the rat. The method is a modification of a technique introduced by Oldendorf and widely used for the study of blood-brain barrier phenomena. While the Oldendorf technique has proven valuable for estimating the relative permeabilities of substances, it is limited in measuring Et under conditions of physiologically or pharmacologically altered permeability or blood flow. The method described in this paper--using a small volume (5 microliters) common carotid injection, a freely diffusible reference tracer, [14C]butanol, and a 5 sec circulation time--allows for measurements of Et that reflect changes in blood flow and small differences in permeability. The modified method is important for the study of the regulation of cerebral vascular permeability and flow in an inexpensive animal model.
本文描述了一种用于定量大鼠脑中扩散受限示踪分子脑血管提取分数（Et）的小体积（5微升）颈总动脉注射方法。该方法是对Oldendorf引入并广泛用于血脑屏障现象研究的技术的改进。虽然Oldendorf技术已被证明在估计物质的相对通透性方面很有价值，但在测量生理或药理改变的通透性或血流条件下的Et时存在局限性。本文所述方法——使用小体积（5微升）颈总动脉注射、可自由扩散的参考示踪剂[14C]丁醇和5秒循环时间——能够测量反映血流变化和通透性微小差异的Et。这种改进方法对于在廉价动物模型中研究脑血管通透性和血流调节具有重要意义。
