Korolev E V, Komissarchik Ia Iu, Filatov S E
Tsitologiia. 1982 Aug;24(8):869-73.
Osmium washing of ultrathin sections of the frog sciatic nerve myelin fibres performed with 4% acetic acid solution preceded their treatment with the Dragendorf solution and ammonium paramolibdate. This enabled us to localize amine and phosphate groups of myelin membranes. The data obtained suggest that the majority of phospholipids of these membranes are located on the cytoplasmic surfaces, whereas glycolipids and cholesterol--on the extracellular ones. Examination of these surfaces by freeze--fracturing revealed different hydratation degrees in these surfaces. These results show that myelin membranes are most convenient models for the study of structural and chemical asymmetry of biological membranes.
用4%醋酸溶液对青蛙坐骨神经髓鞘纤维超薄切片进行锇酸冲洗后，再用Dragendorf溶液和偏钼酸铵处理。这使我们能够定位髓鞘膜的胺基和磷酸基团。所得数据表明，这些膜的大多数磷脂位于细胞质表面，而糖脂和胆固醇位于细胞外表面。通过冷冻断裂对这些表面进行检查，发现这些表面的水化程度不同。这些结果表明，髓鞘膜是研究生物膜结构和化学不对称性的最适宜模型。
