Purz H J, Schlawne M, Büchtemann A, Hartmann J
Acta Histochem Suppl. 1981;23:89-109.
The freeze-etching method is used for investigating the morphology of aqueous polymer systems, e.g. network-like segregation structures of cryofixed polymer solutions and real network structures in polymer gels. The cooling rate, which is decisive for cryofixation of specimens, is measured by a thermocouple and a pyroelectric element in different coolants. The effect of the cooling rate on the freeze-etching structure of polymer solutions, polymer gels and a 10% aqueous glycerol solution as a model substance is determined and compared. The increase of the cooling rate in polymer gels results in maintaining the original network structure, in smaller segregation compartments in polymer solutions and in smaller crystallites in the cryofixed ice matrix.
冷冻蚀刻法用于研究水性聚合物体系的形态，例如冷冻固定的聚合物溶液的网络状偏析结构和聚合物凝胶中的真实网络结构。通过热电偶和热释电元件在不同冷却剂中测量对样品冷冻固定起决定性作用的冷却速率。确定并比较了冷却速率对聚合物溶液、聚合物凝胶以及作为模型物质的10%甘油水溶液的冷冻蚀刻结构的影响。聚合物凝胶中冷却速率的增加导致保持原始网络结构、聚合物溶液中形成更小的偏析区以及冷冻固定的冰基质中形成更小的微晶。
