Bogden J D, Jortner B S, Kemp F W, Zadzielski E
Toxicology. 1981;19(1):21-9. doi: 10.1016/0300-483x(81)90061-5.
Sixty male Swiss-Webster mice were given intraperitoneal injections of the gold-containing drug aurothioglucose in saline at 3 dose levels: 50, 200, and 400 mg/kg. Controls received i.p. injections of saline. Ten injections were administered over a 3-week period. After sacrifice, kidney and liver concentrations of gold, copper, zinc, iron, and manganese were determined by atomic absorption spectrophotometry and tissues were examined by light microscopy. Dose related increases in liver and kidney gold were found and kidney copper also increased significantly with increasing renal gold content. Kidney copper was 5.05 +/- 0.80 ppm in control animals, and 7.81 +/- 1.11 ppm, 13.01 +/- 2.49 ppm, and 17.11 +/- 4.02 ppm in mice receiving 50, 200, and 400 mg/kg aurothioglucose respectively. Renal zinc and liver zinc and copper were also significantly (P less than 0.01) increased in mice receiving the highest dose of aurothioglucose. There were no other statistically significant differences in tissue concentrations among the various groups of animals for the other liver and kidney metal concentrations determined. Tubular epithelial cells of aurothioglucose-dosed animals often had cytoplasmic vacuoles which contained granular brown-gold material; this effect was dose-related.
将60只雄性瑞士韦伯斯特小鼠分为3组，分别腹腔注射含金药物金硫葡萄糖生理盐水溶液，剂量水平为：50、200和400mg/kg。对照组腹腔注射生理盐水。在3周内进行10次注射。处死后，通过原子吸收分光光度法测定肾脏和肝脏中金、铜、锌、铁和锰的含量，并通过光学显微镜检查组织。发现肝脏和肾脏中的金含量与剂量相关增加，并且随着肾脏中金含量的增加，肾脏中的铜含量也显著增加。对照动物的肾脏铜含量为5.05±0.80ppm，接受50、200和400mg/kg金硫葡萄糖的小鼠肾脏铜含量分别为7.81±1.11ppm、13.01±2.49ppm和17.11±4.02ppm。接受最高剂量金硫葡萄糖的小鼠肾脏锌、肝脏锌和铜含量也显著增加（P<0.01）。对于所测定的其他肝脏和肾脏金属含量，各动物组之间的组织浓度没有其他统计学上的显著差异。接受金硫葡萄糖给药的动物的肾小管上皮细胞通常有含有颗粒状棕色金物质的细胞质空泡；这种效应与剂量相关。
