Boyd A W, Schrader J W
J Immunol. 1981 Jun;126(6):2466-9.
WEHI 231 is a murine B cell lymphoma, which from our previous studies is highly susceptible to a lipopolysaccharide (LPS) induced differentiation signal. In this paper we show that anti-immunoglobulin (Ig) antibody at low concentrations (0.1 micrograms/ml) inhibits cell proliferation and results in cell death. Heterologous sera specific for mu- and kappa-chains, and a monoclonal antibody (E4) developed in our laboratory and specific for mu-chain, profoundly suppressed the growth of WEHI 231 in both soft agar culture and liquid culture. The F(ab')2 fragments were as potent as intact Ig, indicating that neither Fc receptor binding nor complement activation were required for this inhibition of growth. Neither heterologous antibodies that bound to WEHI 231 but not to the Ig receptor, nor a monoclonal antibody that bound to non-Ig cell surface structures (a brain-associated antigen) inhibited the growth of WEHI 231. LPS did not prevent inhibition of growth by anti-Ig antibody, and even when addition of LPS preceded the addition of anti-Ig, profound inhibition occurred. WEHI 231 thus promises to be a convenient tool for investigating the mechanism of signal transmission by the Ig receptor.
WEHI 231是一种小鼠B细胞淋巴瘤，根据我们之前的研究，它对脂多糖（LPS）诱导的分化信号高度敏感。在本文中，我们表明低浓度（0.1微克/毫升）的抗免疫球蛋白（Ig）抗体可抑制细胞增殖并导致细胞死亡。针对μ链和κ链的异源血清，以及我们实验室开发的针对μ链的单克隆抗体（E4），在软琼脂培养和液体培养中均能显著抑制WEHI 231的生长。F(ab')2片段与完整Ig的作用效果相同，表明这种生长抑制作用既不需要Fc受体结合，也不需要补体激活。那些与WEHI 231结合但不与Ig受体结合的异源抗体，以及与非Ig细胞表面结构（一种脑相关抗原）结合的单克隆抗体，均不能抑制WEHI 231的生长。LPS不能阻止抗Ig抗体对生长的抑制作用，即使在添加抗Ig之前先添加LPS，也会出现显著抑制。因此，WEHI 231有望成为研究Ig受体信号转导机制的便捷工具。
