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[非特异性复合物形成在蛋白质-核酸识别中的作用]

[Role of nonspecific complex formation in protein-nucleic acid recognition].

作者信息

Bruskov V I, Polozov R V, Poltev V I

出版信息

Biofizika. 1978 Jul-Aug;23(4):610-3.

PMID:678565
Abstract

Combinatory mathematical model of equilibrium binding of recognising proteins to DNA has been considered. The expression for the ratio of specific and unspecific complex as the function of a number of nucleotides in DNA, a number of centers of recognition, concentration of recognising molecules and a code number have been obtained. The analysis of obtained expression for the repressor--operator system showed that binary code would preferable than quartic one. The specificity of binding corresponding to experimental results have been observed by the number of centers more than twenty, in the narrow interval of the energy value and by high limitation on the number of combinations of error centers.

摘要

研究了识别蛋白与DNA平衡结合的组合数学模型。得到了特异性和非特异性复合物比例的表达式,该表达式是DNA中核苷酸数量、识别中心数量、识别分子浓度和编码数的函数。对阻遏物-操纵基因系统所得表达式的分析表明,二进制编码比四进制编码更可取。在能量值的狭窄区间内,通过二十多个中心的数量以及对错误中心组合数量的高度限制,观察到了与实验结果相对应的结合特异性。

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