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[X射线分析在DNP薄膜取向研究中的应用]

[Application of x-ray analysis for the study of the orientation in DNP films].

作者信息

Polivtsev O F, Borshchevskaia T A, Dobretsov G E, Tsvankin D Ia

出版信息

Mol Biol (Mosk). 1975 Jul-Aug;9(4):574-7.

PMID:1214801
Abstract

A planar texture in dried film samples of DNP was found by X-ray study. Axes of DNA macromolecules are located in the film plane and the 77 A reflection is directed along this axis. Periodic structures corresponding to 55 and 34 A are packed parallel to the film plane. The results obtained do not contradict with a supercoil DNP model, however they all allow other possible packing models of DNP molecules, in particular, histone layers alternating with DNA layers in oriented films.

摘要

通过X射线研究发现了二硝基苯酚(DNP)干膜样品中的平面纹理。DNA大分子的轴位于膜平面内,77埃的反射沿此轴方向。对应于55埃和34埃的周期性结构平行于膜平面排列。所获得的结果与超螺旋DNP模型并不矛盾,然而,它们都允许DNP分子的其他可能排列模型,特别是在取向膜中组蛋白层与DNA层交替排列的模型。

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