Renaud J M, Stevens E D
Am J Physiol. 1981 May;240(5):R301-9. doi: 10.1152/ajpregu.1981.240.5.R301.
The effect of acclimation temperature and pH on the isometric twitch and tetanus of sartorius muscle from frog, Rana pipiens, was studied at different experimental temperatures. Seven variables were measured, namely: tension, latent period, time to maximum tension, half-relaxation time, mean rate, maximum rate, and maximum acceleration of tension development. The effect of experimental temperature was similar to that reported in the literature. The effects of acclimation temperature were small and were not compensatory. Different pH's were obtained by varying CO2 in the gas phase, while the HCO3- concentration was kept constant. The main effects of a decrease in pH on the isometric twitch and tetanus were a reduction in tension and rate of tension development and an increase in latent period. A decrease in pH had no effect on the time to maximum tension or the half-relaxation time. Analysis of variance showed that the test temperature had the greatest effect of all three treatments on each variable, the effects of test and acclimation temperature were dependent on neither the test nor the acclimation temperatures. The in vivo relationships between these three treatments are discussed.
在不同实验温度下，研究了驯化温度和pH值对牛蛙（Rana pipiens）缝匠肌等长收缩和强直收缩的影响。测量了七个变量，即：张力、潜伏期、达到最大张力的时间、半松弛时间、平均速率、最大速率和张力发展的最大加速度。实验温度的影响与文献报道相似。驯化温度的影响较小且不具有补偿性。通过改变气相中的二氧化碳来获得不同的pH值，同时使碳酸氢根离子浓度保持恒定。pH值降低对等长收缩和强直收缩的主要影响是张力和张力发展速率降低，潜伏期延长。pH值降低对达到最大张力的时间或半松弛时间没有影响。方差分析表明，在所有三种处理中，测试温度对每个变量的影响最大，测试温度和驯化温度的影响既不取决于测试温度也不取决于驯化温度。文中讨论了这三种处理在体内的关系。
