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[从枯草芽孢杆菌中分离DNA-膜复合物]

[Isolation of DNA-membrane complexes from Bacillus subtilis].

作者信息

Molchanov M I, Nikiforov V V, Trusova V M, Svetlichkin V V

出版信息

Biokhimiia. 1981 Mar;46(3):512-9.

PMID:6786377
Abstract

The DNA-membrane complexes were isolated from the membranes of Bacillus subtilis, disrupted by ultrasonication and subjected to chromatography on Sepharose 4B. The membrane-bound fraction of native 3H-DNA was found only in the freshly isolated material. The phospholipid and protein composition of this fraction was determined. It was assumed that the newly synthesized membrane-associated 3H-DNA from Bacillus subtilis is bound to the lipoprotein complexes enriched with phospholipids.

摘要

从枯草芽孢杆菌的细胞膜中分离出DNA - 膜复合物,通过超声处理使其破碎,然后在琼脂糖4B上进行色谱分析。天然3H - DNA的膜结合部分仅在新鲜分离的材料中发现。测定了该部分的磷脂和蛋白质组成。据推测,枯草芽孢杆菌新合成的与膜相关的3H - DNA与富含磷脂的脂蛋白复合物结合。

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