Duffield P H, Dougan D F, Wade D N, Duffield A M
Biomed Mass Spectrom. 1981 Apr;8(4):170-3. doi: 10.1002/bms.1200080408.
A method has been developed for the quantitation of the putative phenolamine neurotransmitter octopamine, and its precursor tyramine, in brain tissue. The procedure employs methane chemical ionization of the pentafluoropropionate derivatives of octopamine and tyramine together with the use of deuterated internal standards and selected ion monitoring. Deuterated analogues of octopamine and tyramine are added to brain homogenates in aqueous perchloric acid and ion exchange is used to isolate the brain amines. The method is capable of measuring 20 pg of octopamine and tyramine. The measured concentration (ng g-1 wet tissue) of octopamine and tyramine in rat brain was as follows: whole brain (less cerebellum) (0.6 and 2.2); hypothalamus (3.2 and tyramine value not statistically significant); striatum (0.5 and 11.8) and cortex (0.6 and 1.0). Administration of pargyline resulted in an increase (around ten-fold) in octopamine and tyramine concentration in all the above brain regions. In contrast alpha-methyltyrosine produced only a small increase (50%) in the concentration of tyramine in the striatum.
已开发出一种用于定量脑组织中假定的酚胺神经递质章鱼胺及其前体酪胺的方法。该程序采用章鱼胺和酪胺的五氟丙酸酯衍生物的甲烷化学电离，同时使用氘代内标和选择离子监测。将章鱼胺和酪胺的氘代类似物添加到高氯酸水溶液中的脑匀浆中，并使用离子交换来分离脑胺。该方法能够测量20 pg的章鱼胺和酪胺。大鼠脑中章鱼胺和酪胺的测量浓度（ng g-1湿组织）如下：全脑（不含小脑）（0.6和2.2）；下丘脑（3.2，酪胺值无统计学意义）；纹状体（0.5和11.8）和皮质（0.6和1.0）。给予帕吉林导致上述所有脑区中章鱼胺和酪胺浓度增加（约十倍）。相比之下，α-甲基酪氨酸仅使纹状体中酪胺浓度小幅增加（50%）。
