Glode L M, Epstein A, Smith C G
Cancer Res. 1981 Jun;41(6):2249-54.
A murine hybrid cell line has been produced which secretes immunoglobulin G2b with specificity for human gamma-cystathionase (EC 4.2.1.15). The antibody has been iodinated and used in combination with quantitative immunoelectrophoresis in an assay which is capable of detecting as little as 1.5 ng enzyme protein. Human lymphoblastic leukemia cell lines CEM and Laz-221 contain undetectable enzyme protein, corresponding to their behavior as cysteine auxotrophs. In contrast, nonmalignant lymphoblastoid lines contain easily detectable enzyme protein which correlates with their behavior as cysteine prototrophs. Other malignant leukocyte cell lines contained detectable but variable amounts of enzyme protein, suggesting that the enzyme may be a useful marker of cellular differentiation.
已产生一种小鼠杂交细胞系，它能分泌对人γ-胱硫醚酶（EC 4.2.1.15）具有特异性的免疫球蛋白G2b。该抗体已进行碘化，并与定量免疫电泳结合用于一种检测方法，该方法能够检测低至1.5 ng的酶蛋白。人淋巴细胞白血病细胞系CEM和Laz-221不含可检测到的酶蛋白，这与其作为半胱氨酸营养缺陷型的行为一致。相比之下，非恶性淋巴母细胞系含有易于检测到的酶蛋白，这与其作为半胱氨酸原养型的行为相关。其他恶性白细胞细胞系含有可检测但数量可变的酶蛋白，这表明该酶可能是细胞分化的有用标志物。
