Litaker W, Peace-Brewer A, Frelinger J A
Program in Molecular Biology & Biotechnology, University of North Carolina, Chapel Hill 27599, USA.
Mamm Genome. 1996 Mar;7(3):200-5. doi: 10.1007/s003359900054.
The murine class I MHC Q region is part of a large complex multigene family whose members have various peptide binding functions. The structure of the Q region is complex, varying extensively in the b, d, k, and q haplotypes so far examined. To better understand the structural heterogeneity, we examined the Q region of B 10.P, a strain whose immunological characteristics are distinct from other haplotypes. A total of 89 cosmids were isolated from genomic DNA. The B 10.P Q region was found to contain seven genes in a 190-kb cluster linked to DP and two additional Q genes in a separate 55-kb cluster. The gene arrangement in this haplotype was unique and did not correspond to any other haplotype; this underscores the complexity of chromosomal structure in this region. In addition to the Q region clusters, Tla region was tentatively aligned in five clusters spanning approximately 300 kb. One 37-kb M region cosmid was also identified.
小鼠 I 类主要组织相容性复合体 Q 区是一个大型复杂多基因家族的一部分，其成员具有多种肽结合功能。Q 区的结构复杂，在迄今为止所研究的 b、d、k 和 q 单倍型中差异很大。为了更好地理解这种结构异质性，我们研究了 B 10.P 的 Q 区，该品系的免疫特性与其他单倍型不同。从基因组 DNA 中总共分离出 89 个黏粒。发现 B 10.P Q 区在一个与 DP 相连的 190 kb 簇中包含七个基因，在一个单独的 55 kb 簇中包含另外两个 Q 基因。这种单倍型中的基因排列是独特的，与任何其他单倍型都不对应；这突出了该区域染色体结构的复杂性。除了 Q 区簇外，Tla 区初步排列在跨越约 300 kb 的五个簇中。还鉴定出一个 37 kb 的 M 区黏粒。
