Fournier-Delpech S, Colas G, Courot M
C R Seances Acad Sci III. 1981 Feb 16;292(7):515-7.
Prealpes ewes (N = 40) were inseminated with mature (ejaculated or epididymal cauda) or immature (epididymal corpus) spermatozoa deposited in the uterine cavity at the time of an induced ovulation (61 hrs. after PMSG injection). Intratubal eggs, recovered and examined either 38 or 48 hrs. later, showed significant differences in segmentation depending on the degree of epididymal maturation of the spermatozoa. Proportion cleaved: 85% of eggs were cleaved after insemination with mature spermatozoa and only 30% after immature spermatozoa were used. Stage of cleavage: 48 hrs. after insemination with mature spermatozoa, 87% eggs were beyond the 4-cell stage, versus 0% after immature spermatozoa.
对40只前阿尔卑斯母羊进行诱导排卵（注射孕马血清促性腺激素61小时后），在子宫腔内植入成熟（射出或附睾尾）或未成熟（附睾体）精子进行授精。分别在授精38小时或48小时后回收并检查输卵管内的卵子，结果显示，根据精子附睾成熟程度的不同，卵子的分裂情况存在显著差异。分裂比例：用成熟精子授精后，85%的卵子发生分裂，而使用未成熟精子后只有30%的卵子分裂。分裂阶段：用成熟精子授精48小时后，87%的卵子超过4细胞期，而用未成熟精子授精后这一比例为0%。
