Carlsson R, Engvall E, Freeman A, Ruoslahti E
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2403-6. doi: 10.1073/pnas.78.4.2403.
Laminin, a basement membrane glycoprotein isolated from cultures of mouse endodermal cells and rat yolk sac carcinoma cells, promoted the attachment of liver cells obtained from regenerating mouse liver. Cells from normal mouse liver attached readily to dishes coated with fibronectin but attached poorly to surfaces coated with laminin. Both proteins efficiently promoted the attachment of cells from livers undergoing regeneration. After regeneration, the attachment to laminin returned to the low levels found in animals not subjected to partial hepatectomy but attachment to fibronectin remained high. Immunofluorescent staining of sections of normal liver with antilaminin revealed the presence of laminin in or adjacent to the walls of the bile ducts and blood vessels. After induction of regeneration by partial hepatectomy, increased amounts of laminin appeared in the sinusoidal areas. After carbon tetrachloride poisoning, staining for laminin was especially pronounced in the necrotic and postnecrotic areas around the central veins. This additional expression of laminin was transient. It reached a maximum around 5--6 days after the injury and then gradually disappeared. These findings show that laminin is an adhesive protein. The increase of laminin in regenerating liver and the adhesiveness of cells from such livers to laminin suggest a role for laminin in the maintenance of a proper tissue organization during liver regeneration.
层粘连蛋白是从小鼠内胚层细胞和大鼠卵黄囊癌细胞培养物中分离出的一种基底膜糖蛋白，它能促进从小鼠再生肝脏中获取的肝细胞的附着。正常小鼠肝脏的细胞很容易附着在涂有纤连蛋白的培养皿上，但附着在涂有层粘连蛋白的表面上时效果较差。这两种蛋白质都能有效地促进再生肝脏细胞的附着。再生后，细胞对层粘连蛋白的附着恢复到未进行部分肝切除的动物中所发现的低水平，但对纤连蛋白的附着仍然很高。用抗层粘连蛋白对正常肝脏切片进行免疫荧光染色显示，在胆管和血管壁内或其附近存在层粘连蛋白。部分肝切除诱导再生后，肝血窦区域出现了更多的层粘连蛋白。四氯化碳中毒后，中央静脉周围坏死和坏死后期区域的层粘连蛋白染色尤为明显。层粘连蛋白的这种额外表达是短暂的。它在损伤后5 - 6天左右达到最大值，然后逐渐消失。这些发现表明层粘连蛋白是一种黏附蛋白。再生肝脏中层粘连蛋白的增加以及来自这种肝脏的细胞对层粘连蛋白的黏附性表明层粘连蛋白在肝脏再生过程中维持适当的组织组织方面发挥作用。
