Davis P J
Antimicrob Agents Chemother. 1981 Mar;19(3):465-9. doi: 10.1128/AAC.19.3.465.
Seventy-seven microorganisms were examined for their ability to metabolize the antineoplastic agent N-methylcolchiceinamide, an analog of colchicine. Five streptomycetes exhibited significant metabolism, and Streptomyces griseus NRRL B-599 completely converted the substrate to three metabolites. In preparative-scale studies, N-dealkylation resulted in the production of colchiceinamide, the major metabolite (65%), which was characterized by chemical, spectroscopic, and chromatographic comparisons with the standard compound. Two phenolic metabolites resulting from )-dealkylation were also isolated and identified as 2 and 3-O-demethyl-N-methylcolchiceinamide.
对77种微生物代谢抗肿瘤药物N-甲基秋水仙酰胺（秋水仙碱的类似物）的能力进行了检测。5种链霉菌表现出显著的代谢能力，其中灰色链霉菌NRRL B-599能将底物完全转化为3种代谢产物。在制备规模的研究中，N-脱烷基化反应生成了主要代谢产物秋水仙酰胺（65%），通过与标准化合物进行化学、光谱和色谱比较对其进行了表征。还分离并鉴定了由O-脱烷基化产生的两种酚类代谢产物，分别为2-O-去甲基-N-甲基秋水仙酰胺和3-O-去甲基-N-甲基秋水仙酰胺。
