Oğultekin R, Oner M
Mikrobiyol Bul. 1985 Oct;19(4):200-9.
In this work, the methods and technology of vitamin B12 production were studied on laboratory scale. The microorganisms used for experiments were Streptomyces olivaceus IFO 3409, Streptomyces olivaceus CBS 355.53 and Streptomyces griseus CBS 161.45 which were brought from foreign countries. Vitamin B12 activity that have been obtained from fermentation experiments of each microorganisms are determined by microbiological assays using Lactobacillus leichmannii IFO 3073 (ATCC 4797) which is a test microorganism. As a result of these assays S. olivaceus IFO 3409 (2 micrograms/ml) was found as the most efficient strain were followed by S. olivaceus CBS 355.53 and S. griseus CBS 16.45 respectively.
在本研究中，在实验室规模下对维生素B12的生产方法和技术进行了研究。用于实验的微生物是从国外引进的橄榄色链霉菌IFO 3409、橄榄色链霉菌CBS 355.53和灰色链霉菌CBS 161.45。通过使用作为测试微生物的赖氏乳杆菌IFO 3073（ATCC 4797）的微生物测定法，测定从每种微生物的发酵实验中获得的维生素B12活性。这些测定的结果是，发现橄榄色链霉菌IFO 3409（2微克/毫升）是最有效的菌株，其次分别是橄榄色链霉菌CBS 355.53和灰色链霉菌CBS 16.45。
