Kurkus J, Sadowski J, Gellert R, Kruś S
Eur J Clin Invest. 1980 Dec;10(6):463-7. doi: 10.1111/j.1365-2362.1980.tb02086.x.
The role of renal nerves in urine concentration was studied in clearance experiments with unilaterally renal-denervated dogs prepared by surgical bladder division. Urine concentration was assessed under conditions of: (1) stimulation of ADH release by dehydration or barbiturate anaesthesia; (2) mannitol diuresis, and (3) isotonic saline diuresis. Differences in TcH2O/GFR between the intact and denervated kidney were not significant in any of the experimental groups. However, in dehydrated conscious dogs Uosm of the intact kidney (939 +/- 58 mmol/kg H2O) was significantly higher (P less than 0.05) than that of the denervated organ (806 +/- 51 mmol/kg H2O). In the intact kidney the medullary content of total solutes and sodium per kg wet tissue was 684 +/- 26 mmol and 208 +/- 12 mmol, respectively. This was significantly higher (P less than 0.005) than corresponding values of 581 +/- 23 mmol and 171 +/- 14 mmol per kg wet tissue measured in the denervated organ. We suggest that a slight impairment of urine concentration (lower Uosm) in the denervated kidney of conscious dogs may depend on decreased medullary hypertonicity.
通过手术分离膀胱制备单侧肾去神经支配犬的清除实验，研究了肾神经在尿液浓缩中的作用。在以下条件下评估尿液浓缩情况：（1）通过脱水或巴比妥类麻醉刺激抗利尿激素（ADH）释放；（2）甘露醇利尿；（3）等渗盐水利尿。在任何实验组中，完整肾与去神经支配肾之间的自由水清除率（TcH2O）/肾小球滤过率（GFR）差异均不显著。然而，在脱水清醒犬中，完整肾的尿渗透压（Uosm）（939±58 mmol/kg H2O）显著高于（P<0.05）去神经支配肾（806±51 mmol/kg H2O）。完整肾每千克湿组织中总溶质和钠的髓质含量分别为684±26 mmol和208±12 mmol。这显著高于（P<0.005）去神经支配肾每千克湿组织中相应的581±23 mmol和171±14 mmol的值。我们认为，清醒犬去神经支配肾中尿液浓缩的轻微受损（较低的Uosm）可能取决于髓质高渗性降低。
