Semenov Kh Kh, Malashenko A M
Genetika. 1981;17(3):443-8.
Male mice of C57Bl/6Y strain were injected intraperitoneally with 2.5 and 5 mg/kg doses of thioTEPA. Males were mated to tetrahybrid CBWA females during the second week after the treatment. Embryonic mortality was studied by two methods: by standard dominant lethal method on the 15-17th day of pregnancy and cytologically on the 4th day. The rate of fertilization was not affected by thioTEPA. After treatment with 2.5 mg/kg of thioTEPA the frequency of induced dominant lethals was 89.8%; preimplantation losses were 78,5% in treated and 13,8% in control group. The cytological analysis revealed that preimplantation embryonic death is equal to 63,9%. The death of embryos before implantation occurred at 2-20 blastomere stages. After treatment with 5 mg/kg of thioTEPA all embryos died before implantation at 2-16 blastomere stages. It was demonstrated that dominant lethal method gave more complete estimation of dominant lethal frequency, and that cytological analysis is the correct estimation of preimplantation death. Thus the methods used supplement each other.
将2.5毫克/千克和5毫克/千克剂量的噻替派腹腔注射到C57Bl/6Y品系的雄性小鼠体内。在治疗后的第二周，将雄性小鼠与四杂种CBWA雌性小鼠交配。通过两种方法研究胚胎死亡率：在怀孕第15 - 17天采用标准显性致死法，在第4天进行细胞学研究。受精率不受噻替派影响。用2.5毫克/千克噻替派处理后，诱导显性致死的频率为89.8%；处理组着床前损失率为78.5%，对照组为13.8%。细胞学分析显示，着床前胚胎死亡率为63.9%。着床前胚胎死亡发生在2 - 20个卵裂球阶段。用5毫克/千克噻替派处理后，所有胚胎在着床前于2 - 16个卵裂球阶段死亡。结果表明，显性致死法能更全面地估计显性致死频率，而细胞学分析是对着床前死亡的正确估计。因此，所使用的方法相互补充。
