Chambers G K, Laver W G, Campbell S, Gibson J B
Proc Natl Acad Sci U S A. 1981 May;78(5):3103-7. doi: 10.1073/pnas.78.5.3103.
The existence of geographically widespread clines in genetic polymorphisms is persuasive evidence that the distribution of such genetic variance is determined by natural selection. However, when comparing clines it is important to be certain that identical structural genes are involved. We report a structural difference (proline-214 to serine) between the product of AdhF and an electrophoretically cryptic heat-stable variant isolated from an Australian natural population, ADH-FCh.D. ("fast" Chateau Douglas). The biochemical properties of this new variant must be taken into consideration when attempting to formulate a causal explanation of the maintenance of the three identified Adh alleles. Our data also show that the products of an AdhF and an AdhS allele in Drosophila melanogaster in an Australian population are identical over 70% of their amino acid sequences with their North American counterparts.
遗传多态性在地理上广泛存在渐变群，这有力地证明了这种遗传变异的分布是由自然选择决定的。然而，在比较渐变群时，确定涉及相同的结构基因很重要。我们报告了AdhF的产物与从澳大利亚自然种群中分离出的一种电泳隐性热稳定变体ADH-FCh.D.（“快速” 道格拉斯城堡）之间的结构差异（脯氨酸-214变为丝氨酸）。在试图对已确定的三个Adh等位基因的维持做出因果解释时，必须考虑到这种新变体的生化特性。我们的数据还表明，澳大利亚种群中黑腹果蝇的一个AdhF等位基因和一个AdhS等位基因的产物，其氨基酸序列与北美对应物的相同部分超过70%。
