Kojima N, Isemura M, Yosizawa Z, Ono T, Shinada S, Soga K, Aoyagi Y, Ichida F
Tohoku J Exp Med. 1981 Dec;135(4):403-12. doi: 10.1620/tjem.135.403.
Fibronectin was isolated from human plasma by affinity chromatography with gelatin-coupled Sepharose 4B. Anti-fibronectin antiserum was prepared using rabbits, and human liver biopsy specimens at various stages of fibrosis were investigated for distribution of fibronectin by the indirect immunofluorescence technique. Fibronectin was absent in liver parenchymal cells, while it was detected markedly in reticulin and the area containing collagen fibers. The existence of fibronectin in the necrotic area and in prematured fibers suggests the earlier deposition of fibronectin than that of collagen at the locus of tissue repairing, and thus the important role of fibronectin at the early stage of fibrosis.
通过用明胶偶联的琼脂糖凝胶4B进行亲和层析，从人血浆中分离出纤连蛋白。使用兔子制备抗纤连蛋白抗血清，并通过间接免疫荧光技术研究不同纤维化阶段的人肝活检标本中纤连蛋白的分布。肝实质细胞中不存在纤连蛋白，而在网状纤维和含胶原纤维的区域中可明显检测到。坏死区域和新生纤维中纤连蛋白的存在表明，在组织修复部位，纤连蛋白比胶原蛋白更早沉积，因此纤连蛋白在纤维化早期起着重要作用。
