Rinne A, Järvinen M, Räsänen O, Dorn A
Acta Histochem Suppl. 1980;22:325-9.
Using a specific antiserum, it was possible to demonstrate (with the Ouchterlony-test, immunofluorescence and peroxidase-antiperoxidase reaction) that the epidermal thiolprotease inhibitor was present in all the squamous epithelia tested by us, e.g. oesophagus, vagina and portio. Immunological methods further showed that epidermoid carcinoma of the skin, squamoepithelial carcinomas of the portion and oesophagus, and squamoepithelial carcinoma of the lung contained an immunoreactive protein reminiscent of the epidermal protease. The immunoreactive protein typical of squamous epithelium was also visible in part of the anaplastic lung carcinomas, which means that the determination of the inhibitor may be of significance in the classification of lung tumors.
使用一种特异性抗血清，通过免疫双扩散试验、免疫荧光和过氧化物酶-抗过氧化物酶反应能够证明，我们检测的所有鳞状上皮中都存在表皮巯基蛋白酶抑制剂，如食管、阴道和宫颈阴道部。免疫学方法进一步表明，皮肤表皮样癌、宫颈阴道部和食管的鳞状上皮癌以及肺鳞状上皮癌都含有一种免疫反应性蛋白，类似于表皮蛋白酶。在部分间变性肺癌中也可见典型的鳞状上皮免疫反应性蛋白，这意味着该抑制剂的测定在肺肿瘤分类中可能具有重要意义。
