一种适用于自身抗体检测的抗原简易固定法（作者译）
[A simple fixation of antigens suited for the demonstration of autologous antibodies (author's transl)].
作者信息
Gernand K, Rost D, Hanke E, Bent H
出版信息
Acta Histochem Suppl. 1980;22:99-100.
PMID:6789401
Abstract
To determine autoantibodies against nuclear constituents, parietal cells, smooth muscles, mitochondria, microsomes of the thyreoidea and against myocard we use 4 micrometer cryostat tissue sections or monolayer cell preparations. After stepwise desiccation they are shortly heated to 100 degrees C. Antigenicity and histological structures are fully preserved for months, if these substrates were stored at -20 degrees C, provided that rehydration by even traces of moisture is carefully prevented.
摘要
为了检测针对核成分、壁细胞、平滑肌、线粒体、甲状腺微粒体及心肌的自身抗体，我们使用4微米厚的低温恒温器组织切片或单层细胞制剂。经过逐步干燥后，将它们短暂加热至100摄氏度。如果将这些底物保存在-20摄氏度，且小心防止因哪怕微量水分导致的再水化，其抗原性和组织结构可在数月内完全保存。
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